BIOFILM ERADICATION ACTIVITY OF PARTIALLY PURIFIED FRACTION OF ETHYLACETATE EXTRACT OF ACALYPHA WILKESIANA LEAVES ON CANDIDA ALBICANS I, II AND PARAPSILOSIS

Candida spp produce highly structured biofilms composed of multiple cells encased in an extracellular matrix. Sessile are known to decrease drug diffusion toward biological targets. This research is aimed at evaluating the biofilm eradication activity partially purified fraction ethylacetate extract Acalypha wilkesiana leaves on sessile C. albicans I, II and parapsilosis. Column Chromatography-TLC was used fractionate purify using a bioassay guided fractionation. Preparative TLC most potent fraction. Antifungal effects percentage determined by microdilution method. Antibiofilm morphology evaluated scanning electron microscopy. The had MIC 1.25mg/ml parapsilosis, respectively. MFC 2.5mg/ml I but there no total death observed IC50 significantly (p ≤ 0.05) different from that reference drugs. There significant increase eradicated corilagin, amp B., voriconazole, caspafungin, compared biomass parapsilosis all treated plates control. Therefore, A. has potential eradicate formed spp.